By using the McAbs, serum of angiostrongyliasis patient and sera of the rats infected with A. cantonensis were detected by Western blotting and double antibody sandwich ELISA respectively. 使用所得的单克隆抗体以WESTERNBLOTTING检测广州管圆线虫病患者血清,并建立双抗体夹心ELISA法检测感染大鼠血清。
Then, COS-7 cells were transfected through DEAE-Dextran/ chloroquine method. The CD40-Ig fusion protein expressed in COS-7 cell culture supernatant was identified by sandwich ELISA and Western blot. 用DEAEDextran/Chloroquine法转染COS7细胞,夹心ELISA法和Western印迹分析鉴定培养上清中CD40Ig融合蛋白的表达及免疫学活性。